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AICAR ( 50mg )

Table of Contents

  • Quickstart Highlights

AICAR (50mg) protocols center on this AMP-activated protein kinase (AMPK) activator studied for its potential to enhance cellular energy balance, support fat metabolism, and mimic some effects of exercise at the metabolic level. By activating AMPK pathways, AICAR may promote glucose uptake, fatty acid oxidation, and mitochondrial efficiency. This guide outlines a subcutaneous administration approach tailored to the 50 mg vial format.

  • Reconstitute: Add 2.0 mL bacteriostatic water → 25 mg/mL concentration
  • Typical daily range: 10–25 mg per day (subcutaneous)
  • Easy measuring: At 25 mg/mL, 1 unit = 0.01 mL = 0.25 mg (250 mcg) on a U-100 insulin syringe
  • Storage: Lyophilized: store at −20 °C; after reconstitution, refrigerate at 2–8 °C and use within 2–4 weeks
  • Protocol Overview

Concise summary of the subcutaneous regimen.

  • Goal: Support metabolic efficiency through AMPK activation, enhancing fat oxidation and glucose utilization
  • Schedule: Daily subcutaneous injections, often used in cycles
  • Dose Range: 10–25 mg per day
  • Cycle Length: Commonly 2–6 weeks followed by a break
  • Dosing Protocol

Suggested approach for the 50 mg vial format.

  • Start: 10 mg once daily to assess tolerance
  • Target: 20–25 mg once daily
  • Frequency: Once per day (subcutaneous)
  • Vial Duration: One 50 mg vial lasts approximately 2–5 days depending on dose
  • Timing: Morning or pre-activity administration preferred
  • Mechanism of Action

AICAR (5-Aminoimidazole-4-carboxamide ribonucleotide) is an AMP analog that activates AMP-activated protein kinase (AMPK), a key regulator of cellular energy homeostasis.

When AMPK is activated, the body shifts toward energy-producing processes—such as increased glucose uptake and fatty acid oxidation—while reducing energy storage pathways. This results in improved metabolic efficiency, enhanced endurance capacity, and increased mitochondrial activity. AICAR has been widely studied in metabolic and exercise physiology research for its ability to mimic certain biochemical effects of physical activity.

  • Potential Benefits & Side Effects

Observations based on preclinical and early-stage research.

Potential Benefits:

  • May enhance fat metabolism and energy utilization
  • May improve endurance and exercise capacity
  • May increase glucose uptake and insulin sensitivity
  • Supports mitochondrial function and metabolic flexibility

Possible Side Effects:

  • Mild fatigue or low blood sugar sensations in some users
  • Headache or dizziness
  • Injection-site irritation
  • Long-term human safety data remain limited; the compound is investigational